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comstat2  (Oxford Instruments)


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    Oxford Instruments comstat2
    Comstat2, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/comstat2/Imaris/pm32433995-38-25-31
    Average 99 stars, based on 44287 article reviews
    comstat2 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Software:

    Article Title: Optimal surface estimation and thresholding of confocal microscope images of biofilms using Beer's Law.
    Article Snippet: .. There are a number of automatic threshold detection schemes already available in image analysis software such as COMSTAT (implemented in MATLAB) (Heydorn et al., 2000), COMSTAT2 (implemented in ImageJ) (Vorregaard, 2008), Imaris (Imaris, 2018), and daime (Daims et al., 2006). .. Otsu's method for threshold selection, used by the COMSTAT implementations in MATLAB and ImageJ, maximizes the intra-class correlation (Otsu, 1979), where each class is composed of pixels with intensities either above or below the threshold.



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    Compositional analysis and ultrastructural differences in multi-species oral biofilms. Bacterial DNA was extracted from multi- species biofilms using DNeasy Qiagen kit for quantification of each species using SYBR GreenER based quantitative PCR ( A – C ). Unique biofilm morphology and architecture was visualised using scanning electron microscopy (( D – F ); lower magnification ×500, and higher magnification ×5000 inset) and transmission electron microscopy (( G – I ); lower magnification ×15000, and higher magnification ×25000 inset). Confocal stacked images representative of biofilms stained with SYTO9 and PI to show live and dead cells as viewed using the Zeiss LSM 780 confocal laser scanning microscope. Confocal images were taken at ×40 magnification and image stacks compiled using <t>COMSTAT2</t> program. Data for qPCR compositional analysis of biofilms representative of mean +/− SEM for n = 3 from two individual experiments. Abbreviations; A. a* = Aggregatibacter actinomycetemcomitans .
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    Compositional analysis and ultrastructural differences in multi-species oral biofilms. Bacterial DNA was extracted from multi- species biofilms using DNeasy Qiagen kit for quantification of each species using SYBR GreenER based quantitative PCR ( A – C ). Unique biofilm morphology and architecture was visualised using scanning electron microscopy (( D – F ); lower magnification ×500, and higher magnification ×5000 inset) and transmission electron microscopy (( G – I ); lower magnification ×15000, and higher magnification ×25000 inset). Confocal stacked images representative of biofilms stained with SYTO9 and PI to show live and dead cells as viewed using the Zeiss LSM 780 confocal laser scanning microscope. Confocal images were taken at ×40 magnification and image stacks compiled using <t>COMSTAT2</t> program. Data for qPCR compositional analysis of biofilms representative of mean +/− SEM for n = 3 from two individual experiments. Abbreviations; A. a* = Aggregatibacter actinomycetemcomitans .
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    Compositional analysis and ultrastructural differences in multi-species oral biofilms. Bacterial DNA was extracted from multi- species biofilms using DNeasy Qiagen kit for quantification of each species using SYBR GreenER based quantitative PCR ( A – C ). Unique biofilm morphology and architecture was visualised using scanning electron microscopy (( D – F ); lower magnification ×500, and higher magnification ×5000 inset) and transmission electron microscopy (( G – I ); lower magnification ×15000, and higher magnification ×25000 inset). Confocal stacked images representative of biofilms stained with SYTO9 and PI to show live and dead cells as viewed using the Zeiss LSM 780 confocal laser scanning microscope. Confocal images were taken at ×40 magnification and image stacks compiled using COMSTAT2 program. Data for qPCR compositional analysis of biofilms representative of mean +/− SEM for n = 3 from two individual experiments. Abbreviations; A. a* = Aggregatibacter actinomycetemcomitans .

    Journal: Scientific Reports

    Article Title: Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells

    doi: 10.1038/s41598-019-52115-7

    Figure Lengend Snippet: Compositional analysis and ultrastructural differences in multi-species oral biofilms. Bacterial DNA was extracted from multi- species biofilms using DNeasy Qiagen kit for quantification of each species using SYBR GreenER based quantitative PCR ( A – C ). Unique biofilm morphology and architecture was visualised using scanning electron microscopy (( D – F ); lower magnification ×500, and higher magnification ×5000 inset) and transmission electron microscopy (( G – I ); lower magnification ×15000, and higher magnification ×25000 inset). Confocal stacked images representative of biofilms stained with SYTO9 and PI to show live and dead cells as viewed using the Zeiss LSM 780 confocal laser scanning microscope. Confocal images were taken at ×40 magnification and image stacks compiled using COMSTAT2 program. Data for qPCR compositional analysis of biofilms representative of mean +/− SEM for n = 3 from two individual experiments. Abbreviations; A. a* = Aggregatibacter actinomycetemcomitans .

    Article Snippet: Image were taken at x 40 magnification and image stacks compiled using COMSTAT2 program (MATLAB; MA, USA).

    Techniques: Real-time Polymerase Chain Reaction, Electron Microscopy, Transmission Assay, Staining, Laser-Scanning Microscopy